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Snippert, H

Quantitative real-time PCR analysis Total RNA was isolated from 50 mg liver tissue using TRIzol Reagent (Invitrogen, 15596026) with mechanical homogenization (Precellys Evolution, 3 30 s pulses at 6500 rpm), followed by chloroform phase separation (12,000 g , 15 min, 4 C) and isopropanol precipitation

(But, of course, the best way to avoid a hangover is to not drink too much in the first place!) A hangover is typically caused by dehydration and electrolyte imbalances, so it makes sense that infusing fluids and electrolytes directly into the bloodstream through an IV drip could help alleviate some of the symptoms

Zheng Y, Song Y, Han Q, Liu W, Xu J, Yu Z, Zhang R, Li N